Gregory Botting

Experience  Companion diagnostic development for small molecule inhibitors/immunotherapies in NSCLC, melanoma, and head & neck cancer for SnapPath® Cancer Diagnostics System  In vitro and Ex vivo biomarker discovery with cell lines, PDXs and clinical biopsies  Identification of functional signaling profiles of cancer biomarkers for CDx development  Developed manual and automated sample processing methods for isolating live tumor cells from patient biopsies for use with SnapPath® Diagnostics System  Identification of novel drug combinations with anti-cancer synergy (TKIs and various cytokines)  Multiplex ELISA biomarker assay development  Immunotherapy and small molecule drug combination and synergism studies  Robotic instrument validation and troubleshooting in development of SnapPath® System  Training of interns/co-workers in experimental and industry practices  Collaborated with pathologists/oncologists at Johns Hopkins (Baltimore, MD) in obtaining clinical samples and scoring of biomarker profiles  Composition and editing of proposals for successfully funded NCI/NIH grants and provisional patents R&D Techniques and Skills Immunotherapy and small molecule inhibitor in vitro kinetics and titration analysis Cell proliferation assays and optimization Clinical sample biopsy handling; live tumor cell isolation and harvesting ICC/IHC staining of cancer biomarkers in cell lines, PDXs and clinical samples Luminex Multiplex assays and ELISA optimization in biomarker discovery Immunoblotting assays and optimization Operation and optimization of robotic fluidics systems for HTP screening Microscopy and photo imaging Tissue culture and cell line maintenance (development of TKI-resistant cell lines) Small animal model techniques: injections and dissection techniques Adept at Microsoft Office for data analysis and project planning and reporting

Master's Student Researcher