Lois Tolvinski
About
Lois Tolvinski is from Philadelphia, Pennsylvania, United States. Lois works in the following industries: "Biotechnology Research". Lois is currently Manager, Laboratory Operations at Chimeron Bio, located in Philadelphia, Pennsylvania, United States. Lois also works as Scientist at Chimeron Bio, a job Lois has held since Jan 2021. In Lois's previous role as a Associate Scientist at Chimeron Bio, Lois worked in until Jan 2021. Prior to joining Chimeron Bio, Lois was a Scientist II at The Janssen Pharmaceutical Companies of Johnson & Johnson and held the position of Scientist II at Spring House, PA. Prior to that, Lois was a Business Development Intern at The Wistar Institute from Oct 2017 to Jun 2018. Lois started working as Research Assistant IV at The Wistar Institute in Jan 2018. From Jan 2017 to Jan 2018, Lois was Research Assistant III at The Wistar Institute. Prior to that, Lois was a Research Assistant II at The Wistar Institute, based in Greater Philadelphia Area from Jan 2015 to Jan 2017. Lois started working as Research Assistant I at The Wistar Institute in May 2013.
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Lois Tolvinski's current jobs
ChaESAR Nanoparticle Production Lead Upstream processing
Lois Tolvinski's past jobs
Kelly Services BPO In vitro pharmacological compound profiling for multiple projects supporting lead discovery and hit-to-lead programs for Oncology and Immunology therapeutic areas Biochemical and cell-based assay development in microplate format (384/1536) Quantitative data analysis and timeline-based data delivery to enable medicinal chemistry SAR studies Cross sector collaboration: Naturals platform, Emerging Science and Innovation
Identifying tangible materials and enabled inventions in unpublished manuscripts; presenting findings to team of interns and business development team Due diligence research Learning the process of developing an intellectual property portfolio Developing an internal blog detailing the work happening at the intersection of business and science
Lab Manager Assist in drafting study sections of IND Developing and optimizing an in vitro target engagement study using UV-activated crosslinking compounds
Lab manager Write and review pre-IND study reports on AlphaScreen and HTRF biochemical assays In vivo studies using GFP and luciferase in an EBV+ tumor mouse model; including tumor implantation, IP injections, oral gavage, live animal imaging, and anesthesia use IVIS Spectrum imaging: 2D, 3D, and micro-CT Software: Living Image for IVIS image acquisition and analysis
Lab Manager Conduct biochemical high throughput screening assays AlphaScreen and HTRF assessing compound disruption of protein/DNA interaction Protein expression in e.coli and purification using affinity chromatography and size-exclusion chromatography Software: Dotmatics for analysis and databasing results; GraphPad Prism
Compound library management of 3000+ novel small molecules synthesized by medicinal chemist collaborators Molecular biology techniques; protein expression and purification, western blotting, SDS-PAGE electrophoresis, cloning