Patricia Lin
About
Patricia Lin is from Portland, Oregon, United States. Patricia works in the following industries: "Wellness and Fitness Services". Patricia is currently Quality Control & Research Assistant at UbiVac. In Patricia's previous role as a Laboratory Technician at Biologic Resources LLC, Patricia worked in until Aug 2021. Prior to joining Biologic Resources LLC, Patricia was a Specimen Processor at ZRT Laboratory and held the position of Specimen Processor. Prior to that, Patricia was a Seasonal Cashier at Columbia Sportswear Company, based in Beaverton, OR from Oct 2018 to Dec 2018. Patricia started working as Classroom Undergraduate Research Experience (CURE) at Oregon State University in Apr 2018.
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Patricia Lin's current jobs
Patricia Lin's past jobs
- Made and reshelved broth and agar media in autoclave. Routinely cleaned and sanitized autoclaves, incubators, benches, trash bins, communal areas, and media bottles. - Sorted and sampled product and environmental samples for pathogen testing. Ran next-day and two-day samples on Biomerieux VIDAS as per lab SOPs and logged results for various clients.
- Received, opened, and sorted 600-1200 samples/day, primarily human saliva, dried blood, dried urine hormone & metabolite testing kits. - Accessioned, labeled, and logged patient and sample information from requisitions into custom software
Supported front end team to provide quality customer service to a reach of 1,000 visitors per day at Columbia Sportswear's Employee Store. Trained in POS system, store fulfillment, floor restocking, and fitting room management.
Molecular Microbiology Lab topics: 1. CRISPR-Cas9/Recombineering Mutagenesis to culture Streptomycin-resistant E. coli strain - Electroporated E. coli to introduce a single-stranded λ-phage-driven recombineering oligonucleotide housed in circular plasmid DNA - Observed oligonucleotide mutagenesis across different dilutions on chloramphenicol, kanamycin, and streptomycin-treated plates. - Analyzed agarose gel electrophoresis and nanodrop spectrometer results to check purity of plasmid DNA and PstI-digested plasmid DNA. - Used Qubit fluorometer and nanodrop spectrometer to measure concentration of purified amplicons - Analyzed Sanger sequenced rpsL genes from OSU Center for Genome Research and Biocomputing (CGRB lab) with breseq and blastn - Verified presence of K43T mutations in electroporated strains with activated recombineering genes and found unexpected K43R mutation in a oligonucleotide-less, CRISPR-less strain. 2. Census of Uncultured Microbial Communities in Solarized Soils - Extracted 16S rRNA genes from solarized soil provided by Dr. Jennifer Parke of the W-SARE project for PCR and Illumina MiSeq DNA sequencing. - Assessed purification of PCR product using results from agarose gel electrophoresis, Qubit fluorometer, and nanodrop spectrometer. - Analyzed Illumina MiSeq data from OSU Center for Genome Research and Biocomputing (CGRB lab) with mothur - Compared species richness and alpha diversity between multiple samples and plot similar communities using non-metric multidimensional scaling (MDS), PRIMER software, and the Shannon diversity index. - Concluded solarized soil with high moisture in soil may result in increase of taxa unrelated to abundant community members Acidobacteria, Burkholderiales, and Sphingobacteriales.