Satish Adhikari
About
Satish Adhikari is from Philadelphia, Pennsylvania, United States. Satish works in the following industries: "Biotechnology Research". Satish is currently Scientist at Chimeron Bio, located in Philadelphia, Pennsylvania, United States. In Satish's previous role as a Research Assistant at University of Mississippi, Satish worked in Oxford, Mississippi, United States until Dec 2021. Prior to joining University of Mississippi, Satish was a Graduate Teaching Assistant at University of Mississippi and held the position of Graduate Teaching Assistant at Oxford, Mississippi, United States. Prior to that, Satish was a Research Associate at Research Institute for Bioscience & Biotechnology (R.I.B.B), based in Lalitpur District, Nepal from Dec 2011 to Jul 2014.
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Satish Adhikari's current jobs
Satish Adhikari's past jobs
• Routinely used Gibson Assembly to create constructs for various purposes such as recombinant protein production, mutant generation and reporter assays. • Extensively used affinity chromatography and size exclusion chromatography to purify recombinant proteins for antibody production, gel shift assay. • Used Next Generation Sequencing methods such as RNA-seq and ChIP-seq to study global gene expression profile. Used RT-qPCR to verify the global gene expression profile. • Performed immunoprecipitation (IP) and mass spectrometry (MS) to study protein-protein interaction. Used bacterial two hybrid assay to verify the interaction. • Used confocal microscopy to study gfp and mcherry tagged protein localization. • Mentored undergraduate students in their final year thesis.
• Taught several laboratory courses to undergraduate students. • Gained invaluable experience in mentoring students with various needs and learning abilities. • Guest lecturer in advanced microbiology class for undergraduate students.
• Involved in setting up in a new research lab. Responsibilities included optimizing protocols for several new equipments. • Trained new employees on using the lab equipment such as thermal cycler, gel electrophoresis unit, SDS PAGE. • Involved in isolation and characterization of novel yeast strains. • Involved in several projects with varying responsibilities including designing and performing experiments. Other responsibilities include writing, and presenting lab reports and supervising junior researchers.