Sophie Porret
About
Sophie Porret is from Cowley, England, United Kingdom. Sophie works in the following industries: "Biotechnology Research". Sophie is currently Principal Scientist at SpyBiotech, located in Cowley, England, United Kingdom. In Sophie's previous role as a Senior Scientist - Upstream and Downstream Process Development at SpyBiotech, Sophie worked in Oxford, United Kingdom until Aug 2020. Prior to joining SpyBiotech, Sophie was a Downstream Process Development Research Scientist at UCB and held the position of Downstream Process Development Research Scientist at Slough, Royaume-Uni. Prior to that, Sophie was a In Process Control Scientist at Amaris, based in Région de Bruxelles, Belgique from Oct 2014 to Dec 2015. Sophie started working as Cell Culture Scientist at PX'Therapeutics, SA, Grenoble, France in Région de Grenoble, France in Jan 2013. From Sep 2012 to Jun 2013, Sophie was Master's Degree Internship at PX-Therapeutics, based in Grenoble. Prior to that, Sophie was a Technician Quality Control at PX'Therapeutics, SA, Grenoble/Lyon, France, based in Région de Grenoble, France from Dec 2007 to Sep 2012. Sophie started working as Technicien analyste at TERA Environnement in Crolles, Isère, France in Oct 2006.
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Sophie Porret's current jobs
Lead HCMV antigen candidate process development (USP/DSP), scale up and transfer to GMP facility. Lead the HBsAg VLP production and GMP process transfer
Sophie Porret's past jobs
Lead HCMV antigen candidate process development (USP/DSP), scale up and transfer to GMP facility.
Environment: Development of downstream processes for the recovery and purification of antibody-based therapeutics. The role consists in execute process understanding and optimisation for drug coming up to Clinical Phase III studies. Understand impact of process on product, explore operating ranges, push process to its limits and implement changes that improve robustness. - Undertake purification, UFDF process development experiments for fundamental understanding on AKTA device (AVANT, Purifier, TECAN, CrossFlow, Flux) - Identify and utilise appropriate analytical techniques - Ability to analyse data, with good attention to detail (MODDE, DoE) - Communicate findings to multi-disciplinary Process Development Teams
Environment/Context: Biologic Pilot Plant - Manufacturing Biological Drug Substance for human use - Multiproduct facility Role: Operational and analytical support - timely monitoring of the GMP and non-GMP process - understanding of the manufacturing process and relevant product quality attributes. -Purity by Bioanalyseur/SDS-PAGE -DNA Clearance by qPCR -Size variants by SE-HPLC -Protein A/HCP assay by ELISA -mAbsTitre by ProtA HPLC -etc... LIMS Samples management Equipment Project management Batch Record Review Contribute the logistic in the lab Yellow Belt Certification
Production of recombinant protein in mammalian cell: - transient transfection (PEI - Fectin ...), - selection of the best cell line (CHO, HEK) and productivity evaluation by performing expression test (SDS-PAGE, Western Blot, ELISA, activity test....), - smal scale purification assays (screening IEX or HIC ... chromatography on AKTA Explorer, Labscale) - Scale up, batch production (flask, bioreactor)
Lentivirus vector technology evaluation applied to recombinant protein production. Evaluation on CHO-S and HEK cell lines (cloning, stable cell line), USP (flask) - DSP (3 steps purification on AKTA) and quality control on protein of interest (activity test: Clot Lysis, Western Blot, SDS-PAGE, ELISA, SE-HPLC, N-ter sequencing).
Technician in a Quality Control department of Biotecnology company, PX'Therapeutics (Grenoble - France). Stability control on final product involved in preclinical and clinical trials. Protein production from bacterial and cell culture in flask. Analytical method development. Method used: ELISA, Western Blot, SDS-PAGE, Bradford, Endotoxin test (LAL), HPLC, Protein and antibody purification (AKTA), microbiologic analysis (viability, plasmidic retention, cell and plasmid identification ...)
Analyse qualitative et quantitative de prélèvement atmosphérique par spectrométrie de masse (ATD-GC-MS).
Optimization of the 2D LC/MS/MS technique for protein identification. First dimension: -Sample pretreatment (proteins extraction from biomass/Tryptic digestion) -1D SCX liquid chromatography system, optimization of buffer and gradient Second dimension: -Pretreatment, fraction desalting (Waters Oasis HLB column) -2D RP C18 trap column/ RP C18 analytical column/ ESI ionization technique/ Q-ToF or LCQ mass analyzer -Research of the best MS method, optimization of the elution/fraction time -Identification using database searching (MASCOT/BIOWORKS)