Sweta Raval

• Led and performed original research project: Small molecule screening against human orphan G protein-coupled receptors (GPCRs) expressed in the striatum of the brain, with the goal of identifying novel agonists and antagonists for these new receptors. o Optimized a high throughput PRESTO-Tango beta-arrestin assay and screened two large chemical libraries against five orphan GPCRs: GPR6, GPR52, GPR88, GPR101 and GPR149. Identified and validated several novel agonist ligands for GPR88 and GPR149. o Determined orphan receptor G protein coupling mechanisms and signaling via Gs/olf, Gi/o or Gq/11 and activation/inhibition of secondary messenger signaling cascades (cAMP, IP1 and calcium). • Collaborated and performed research on several other projects including: o NMU2 receptor signaling studies including dose-response assays to determine potency and efficacy of NMU2R selective agonist ligands on cAMP and beta arrestin signaling. o Studied 5-HT2A/2C-mediated calcium signaling via Gq/11 proteins or arrestins in wildtype cells and knockout cell lines, formed using CRISPR/Cas9 genome editing. o Performed dose response curves of novel D1 receptor ligands on cAMP and beta arrestin signaling. Also, studied D1R mediated cAMP signaling via Gs/olf proteins in wildtype and knockout cell lines.

Performed quantitative determination of HCP in the samples purified from CHO cells using ELISA Conducted DNA extraction/purification and analysis of residual CHO genomic DNA in samples using QPCR Determined concentration of antibodies using A280 method Well trained in assays like CEX, N-glycan extraction and derivatization, Peptide mapping Well trained in handling Tecan mediated liquid filling Well-versed with making and maintaining electronic notebook and sharing data using LIMS

Whole body autoradiography: Carried out polishing of the mouse slices for phosphorimaging and performed analysis using radioactivity counts obtained in different regions of the mouse body Cell-culture: Performed the culture of N27 cells (immortalized rat mesencephalic cells) and worked on morphometric analysis of them using a semi-automated analytical program ImageJ Immunocytochemistry: Performed immunocytochemistry of N27 cells for detecting proteins called synaptophysin and BDNF followed by microscopy and image analysis HPLC: Optimized and ran HPLC for DHA, synaptamide and phospholipids using different solvent systems Radiometric data analysis: Determined radioactivity in various tissues of mice which were injected with various radioactive drugs (14C and 125I labeled) using liquid scintillation and gamma counter respectively

In vitro techniques: Successfully performed genotyping of transgenic mice using mouse tails. Carried out DNA extraction using mouse tail and PCR analysis followed by agarose gel electrophoresis to determine genotype of the mouse. Learnt to keep proper records and troubleshooting to obtain perfect research data Worked on Immunohistochemistry of brain tissues like striatum and nucleus accumbens for detecting expression of c-Fos while understanding the use of optogenetic techniques in regulating adenosine receptor expression In vivo techniques: Observed mouse dissection and brain perfusion, brain tissue sectioning and surgery in mouse brain of incorporating channel rhodopsin in the brain region of interest through a cannula

I performed manufacturing of solid dosage forms following process validation protocol I observed packaging and labeling under cGMP conditions