Harindra Sathkumara Ph D

-Currently manages a team of Application Specialists by providing technical and intellectual support in the context of COVID-19 and other molecular research and diagnostics -Responsible for initiating new medical and life-science related projects with the primary focus on local manufacturing in post-COVID era.

Detection of genotypes and serotypes of Leptospira isolated from severe leptospirosis patients -Real time PCR based detection of Leptospira from patients' samples -MLST based genotyping of isolated Leptospira strains -Whole genome sequencing of severe disease causing local Leptospira strains

Development of Course Material in Bachelor of Medical Laboratory Science Honours Degree Program -Flow Cytometry (CD markers and Immunophenotyping) -Reference Range Calculation

-Curriculum development on Blood and Immune module -Curriculum development on Community Medicine

Studied the mechanistic aspects of tuberculosis susceptibility and vaccine efficacy in type-2 diabetes and HIV. -Safety, immunogenicity and efficacy of novel experimental recombinant vaccines against pulmonary TB being assessed using robust animal models of T2D and HIV (PC3 or Biological safety level-III; BSL-3) -Techniques used: in vivo, in vitro and ex vivo infections, genetically engineered recombinant BCG strains, primary cell cultures (BMDCs and BMDMs), T cell proliferation assays, multiplex immune assays, high- dimensional NanoString assays, high-throughput 16S RNA Seq (microbiome), multi-colour FACS

− Project Management of the current research studies at the institute. − Carrying out certain molecular and immunological assays (Blood sample processing, PBMC isolation, DNA extraction, PCR, Real Time qPCR, ELISA, Sanger Sequencing, Next-Gen Sequencing, Phylogenetics, Statistics & data analysis). − Coordinating clinical samples from different stations. − Maintenance of a smooth laboratory workflow.

-Act as the main line of communication between the sponsor and the investigator Verifying that all research staff, facilities and investigational products have adequate qualifications and resources and these remain adequate throughout -Responsible for the safety and proper conduct throughout the trial -Verifying that the investigator follows the approved protocol and all GCP procedures -Verifying that source data/documents and other trial records are accurate, complete, and maintained Adverse events, concomitant medications, and inter current illnesses are reported in accordance with the protocol on the CRFs -Communicating deviations from the protocol, SOPs, GCP, and the applicable regulatory requirements to the investigator

-Collect specimens from operating theatres, out-patients clinics, the clinical laboratory office, and other points of specimen collection within the hospital as requested. -Maintain a supply of specimen containers and specimen pots containing fixative within the department and in the operating theatres. -To supply other Trust locations and other Hospital Trusts with containers and specimen pots on demand. -Assist the pathologist at cut up. Match specimens with requests, number specimens and request forms, label cassettes, place specimens in cassettes and fixative. -Record specimen details on paper or on computer. Maintenance of Histological/Cytological equipment, preparation of stains and reagents, and the upkeep of laboratory areas.

-Introduction to one of the busiest medical laboratory environment. -Continuous use of peer reviewed medical journals, both paper & electronic. -Responsible with over 40 patients who have been attending to the lipid clinic regularly and their medical records. -Identify abnormalities of laboratory test results and their causes. -Scientific approach to the genesis of the diseases across the basic medicines. -The laboratory data were analysed utilising the Microsoft Excel & SPSS packages. -Data protection regulations were maintained over the six months of the placement.